Stable-isotopic and metagenomic analyses reveal metabolic and microbial link of aerobic methane oxidation coupled to denitrification at different O2 levels
نویسندگان
چکیده
Aerobic methane (CH4) oxidation coupled to denitrification (AME-D) can not only mitigate CH4 emission into the atmosphere, but also potentially alleviate nitrogen pollution in surface waters and engineered ecosystems, it has attracted substantial research interest. O2 concentration plays a key role AME-D, yet little is understood about how impacts microbial interactions. Here, we applied isotopically labeled K15NO3 13CH4 metagenomic analyses investigate metabolic link of AME-D at different levels. Among four experimental levels 21%,10%, 5% 2.5% 8% (i.e., O2/CH4 ratios 2.62, 1.26, 0.63 0.31), highest NO3?-N removal occurred system incubated 10%. Methanol acetate may serve as trophic linkage between aerobic methanotrophs denitrifers systems. Methylotrophs including Methylophilus, Methylovorus, Methyloversatilis Methylotenera were abundant under O2-sufficient condition with 21%, while denitrifiers such Azoarcus, Thauera Thiobacillus dominated O2-limited environment The competition methylotrophs for CH4-derived carbon, methanol acetate, might be influenced by chemotactic responses. More methane-derived carbon flowed condition, more was used environment. These findings aid evaluating distribution contribution developing strategies mitigating natural ecosystems.
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ژورنال
عنوان ژورنال: Science of The Total Environment
سال: 2021
ISSN: ['0048-9697', '1879-1026']
DOI: https://doi.org/10.1016/j.scitotenv.2020.142901